ISee! is able to perform a so-called "flat field" correction as well as the detection and the correction of bad pixels. 

In order to do it:

1. A sequence of uniformely illuminated images has to be taken as the first step.
   They are called correction or reference images. The exposures have to be done without an object.
   All setup-variables have to be the same as with the object, except the number of frames to be 
   integrated - it has to be 3-5 times higher for the calibration images.
   Mean intensities of these images have to range from the minimum till the maximum representable value, 
   i.e. cover whole dynamic of the detector.

2. Create a configuration file. Essentially it is a list of reference images, stored as plain ASCII-text, 
   one filename per line, ordered from the darkest (dark image) to the brightest. It is used by "ISee!" 
   for fine adjusting of the grey values of individual pixels in real (i.e. with object) shots. 
   If an acquisition system is inherently and highly linear, then only two (black and white) correction images 
   are enough. Otherwise (for a nonlinear system) more correction images are necessary since 1st order (linear) 
   interpolation is used between corrected grey values. An extensive example of a configuration file with all 
   options explained is in "hamamatsu\correction-1m-nofilter-120kV\ccorrection-all-in-one.txt".
